Liverpool captain Henderson picked up a knock in Sunday’s defeat against Belgium while Manchester City forward Sterling is withdrawing with a calf complaint.
The pair have returned to their respective clubs for further assessment.
England boss Gareth Southgate will continue his preparations for the Wembley encounter, live on Sky Sports, with a 22-man squad.
Jordan Henderson picked up a knock in Sunday’s defeat against Belgium
Henderson was withdrawn at half-time against Belgium due to tightness in his leg, casting doubt on his availability for injury-hit Liverpool this weekend.
Liverpool face Leicester at Anfield on Sunday, live on Sky Sports.
“Jordan had a bit of tightness at half-time and felt he couldn’t carry on,” said Southgate.
Manchester City travel to Tottenham on Saturday and could be without Sterling, who suffered a “small” injury ahead of the Belgium game.
Speaking before kick-off, Southgate said: “Raheem felt something a couple of days ago and didn’t train on Friday as a precaution.
“He started training on Saturday and wasn’t really happy with it. We have discussed it and it isn’t one to take a chance on.”
